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Environmental DNA (eDNA)

Santhosh Kumar 04/11/2023

Environmental DNA (eDNA) mind map
News/Context
Scientist estimated total taxonomic diversity
Chilika lagoon in Odisha
About 1,071 families
Across the tree of life
Using environmental DNA (eDNA) method
What
eDNA
Definition
Nuclear or mitochondrial DNA
Released into the environment
During organism's lifetime or after death
Sources of eDNA
Secreted feces
Mucous
Gametes
Shed skin and hair
Carcasses
eDNA method
Non-invasive method
Assess total biodiversity
By sequencing DNA
Found in the environment
Developed by
LaCONES
Full form
Laboratory for the Conservation of Endangered Species
Part of
CSIR-Centre for Cellular and Molecular Biology
Headquartered in Hyderabad
Sample size
Few litres of
Water
Soil
Air
Working mechanism
Molecular approach
Filter out eDNA from samples
Read sequences
Identify eDNA source
Techniques used
PCR
Full form
Polymerase chain reaction
Purpose
Amplify DNA for sequencing
qPCR
Full form
Quantitative PCR
Purpose
Detect and quantify DNA
Why
Significance
Cheaper
Faster
Highly scalable
Applications
Monitoring ecosystems
Conserving biodiversity
Detecting various organisms
Viruses
Bacteria
Archaea
Eukaryotes
Revealing presence
Human remains
Where
Chilika lagoon in Odisha
Country's largest brackish water lagoon
Plane wreck vicinity
2nd World War
For floating eDNA
Sample collection
Seawater
When
Recent estimation
Total taxonomic diversity
Who
Scientists
Method application
eDNA to estimate diversity
LaCONES
Development of eDNA method
How
Collecting samples
From various environments
Water
Soil
Air
Using PCR and qPCR
For DNA sequencing

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Categories: Biotechnology

Tags: 4.11.2023, Newsbits

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