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Environmental DNA (eDNA)

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Santhosh Kumar 04/11/2023
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Environmental DNA (eDNA) mind map News/Context Scientist estimated total taxonomic diversity Chilika lagoon in Odisha About 1,071 families Across the tree of life Using environmental DNA (eDNA) method What eDNA Definition Nuclear or mitochondrial DNA Released into the environment During organism's lifetime or after death Sources of eDNA Secreted feces Mucous Gametes Shed skin and hair Carcasses eDNA method Non-invasive method Assess total biodiversity By sequencing DNA Found in the environment Developed by LaCONES Full form Laboratory for the Conservation of Endangered Species Part of CSIR-Centre for Cellular and Molecular Biology Headquartered in Hyderabad Sample size Few litres of Water Soil Air Working mechanism Molecular approach Filter out eDNA from samples Read sequences Identify eDNA source Techniques used PCR Full form Polymerase chain reaction Purpose Amplify DNA for sequencing qPCR Full form Quantitative PCR Purpose Detect and quantify DNA Why Significance Cheaper Faster Highly scalable Applications Monitoring ecosystems Conserving biodiversity Detecting various organisms Viruses Bacteria Archaea Eukaryotes Revealing presence Human remains Where Chilika lagoon in Odisha Country's largest brackish water lagoon Plane wreck vicinity 2nd World War For floating eDNA Sample collection Seawater When Recent estimation Total taxonomic diversity Who Scientists Method application eDNA to estimate diversity LaCONES Development of eDNA method How Collecting samples From various environments Water Soil Air Using PCR and qPCR For DNA sequencing

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